Cigarette Smoke Induces DNA Deletions in the Mouse Embryo1
نویسندگان
چکیده
Cigarette smoking causes cancer and DNA mutations. However, longterm chronic exposure to smoke is believed to be necessary for carcinogenesis. Here, we investigate the relationship between short-term exposure to smoke and the frequency of deletions in the mouse embryo. Deletions and other genome rearrangements are associated with carcinogenesis and inheritable diseases. The pink-eyed unstable (/>"") mutation in the C57BL/6J mouse is the result of internal duplication of 70 kb of DNA within the p gene. Spontaneous reversion events in homozygous p""/p"" mice occur by deletion of one copy of the duplicated sequence. Reversion events occurring in the embryonic premelanocytes of the developing fetus give rise to black spots on the gray fur of the offspring after birth. We investigated the effects of exposure of pregnant p°"mice to cigarette smoke and cigarette smoke condensate (CSC) on the frequency of black spots occurring in the offspring. Pregnant dams were exposed (whole body) to smoke generated by either filtered or unfiltered cigarettes for 4 h, or alternatively, mice were given a 15 mg/kg dose of CSC during their 10th day of gestation. I I'M. CO concentration, and plasma nicotine and cotinine levels were determined to characterize the smoke exposure. There was a significant increase in the number of DNA deletions in the embryo as evidenced by spotted offspring in both smoke-exposed groups and in the CSC group. These results suggest that embryos are highly sensitive to the genotoxic activity of cigarette smoke following a single exposure of only 4 h.
منابع مشابه
Cigarette smoke induces DNA deletions in the mouse embryo.
Cigarette smoking causes cancer and DNA mutations. However, long-term chronic exposure to smoke is believed to be necessary for carcinogenesis. Here, we investigate the relationship between short-term exposure to smoke and the frequency of deletions in the mouse embryo. Deletions and other genome rearrangements are associated with carcinogenesis and inheritable diseases. The pink-eyed unstable ...
متن کاملToxicity Mechanisms of Cigarette Smoke on Mouse Fetus Mitochondria
Maternal smoking has been recognized as a common cause of low birth weight, preterm birth and the decrease of gestational age period. Unfortunately, there is an increasing interest within public especially woman in Iran in the tobacco products consumption. On the other hand, the deleterious effect of maternal smoking on human fetus in pregnancy period especially in the first trimester encourage...
متن کاملToxicity Mechanisms of Cigarette Smoke on Mouse Fetus Mitochondria
Maternal smoking has been recognized as a common cause of low birth weight, preterm birth and the decrease of gestational age period. Unfortunately, there is an increasing interest within public especially woman in Iran in the tobacco products consumption. On the other hand, the deleterious effect of maternal smoking on human fetus in pregnancy period especially in the first trimester encourage...
متن کاملCIGARETTE SMOKE/ETHANOL-INDUCED LIMB DEFECTS IN MOUSE EMBRYOS
We have produced a spectrum of limb defects in developing mouse embryos by exposing the mother to smoke from cigarettes with different nicotine concentrations, ethanol, and a combination of ethanol and cigarette smoke. The critical time of exposure was determined to be during day 10 of gestation (vaginal plug=day 0). This time is prior to the critical events which occur between the apical e...
متن کاملChinese green tea consumption reduces oxidative stress, inflammation and tissues damage in smoke exposed rats
Objective(s):One cause of cigarette smoking is oxidative stress that may alter the cellular antioxidant defense system, induce apoptosis in lung tissue, inflammation and damage in liver, lung, and kidney. It has been shown that Chinese green tea (CGT) (Lung Chen Tea) has higher antioxidant property than black tea. In this paper, we will explore the preventive effect of CGT on cigarette smoke-in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2006